Whey fermentation: on-line analysis of lactose and lactic acid by FTIR spectroscopy.
- 1 June 1991
- journal article
- Published by Springer Nature in Applied Microbiology and Biotechnology
- Vol. 35 (3), 301-305
- https://doi.org/10.1007/BF00172716
Abstract
The batch fermentation of whey permeate by Lactobacillus helveticus 8652, was monitored on-line by Fourier transform infrared (FTIR) spectroscopy. Substrate (lactose) and product (lactic acid) levels were measured over a period of 47 h. A method for the quantitative analysis of the two components was first established. Fifteen standard solutions containing both lactose and lactic acid were used. The method developed was tested using validation samples of known composition. Mean errors of 1.1% and 0.9% were attained in the measurement of lactose and lactic acid respectively. Sample analysis is fast (approx. 3 min), simple and almost completely automated. The results obtained with FTIR spectroscopy compared favourably with samples analysed off-line using enzyme kits.Keywords
This publication has 15 references indexed in Scilit:
- On-line determination of lactic acid during kefir fermentation based on a fibre-optic lactic acid biosensor and flow-injection analysisAnalytica Chimica Acta, 1990
- Flow injection analysis of L-lactate with enzyme amplification and amperometric detectionAnalytical Chemistry, 1990
- Multivariate calibration for assays in clinical chemistry using attenuated total reflection infrared spectra of human blood plasmaAnalytical Chemistry, 1989
- Multivariate determination of glucose in whole blood by attenuated total reflection infrared spectroscopyAnalytical Chemistry, 1989
- Fermentation of cheese whey–monitoring by FT-IRAnalytical Proceedings including Analytical Communications, 1989
- In Situ FT-IR Analysis of a Composite Curing Reaction Using a Mid-Infrared Transmitting Optical FiberApplied Spectroscopy, 1988
- Partial Least-Squares Quantitative Analysis of Infrared Spectroscopic Data. Part II: Application to Detergent AnalysisApplied Spectroscopy, 1988
- Consistency in Circle Cell FT-IR Analysis of Aqueous SolutionsApplied Spectroscopy, 1987
- Quantitative Fourier Transform Infrared Methods for Real Complex SamplesPublished by ASTM International ,1987
- A MEDIUM FOR THE CULTIVATION OF LACTOBACILLIJournal of Applied Bacteriology, 1960