Expression of antigenic determinants of the hemagglutinin gene of a human influenza virus in Escherichia coli.
- 1 September 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (9), 5376-5380
- https://doi.org/10.1073/pnas.78.9.5376
Abstract
Antigenic determinants of influenza virus hemagglutinin were expressed in Escherichia coli. DNA coding for presequences of hemagglutinin were removed and an ATG codon was placed before DNA coding for mature hemagglutinin. A number of expression plasmids were constructed in which various segments of this reconstructed hemagglutinin DNA were fused to DNA coding for bacterial beta-galactosidase. The fusion proteins exhibited specific binding to antiviral antibodies. This binding could be competitively inhibited by excess viral hemagglutinin, demonstrating that these fusion proteins contained antigenic determinants of hemagglutinin.This publication has 22 references indexed in Scilit:
- Complete sequence analysis shows that the hemagglutinins of the H0 and H2 subtypes of human influenza virus are closely relatedVirology, 1981
- Antigenic determinants of influenza virus hemagglutinin II. Antigenic reactivity of the isolated N-Terminal cyanogen bromide peptide of A/memphis/72 hemagglutinin heavy chainVirology, 1979
- STRUCTURE OF THE HAEMAGGLUTININ OF INFLUENZA VIRUSBritish Medical Bulletin, 1979
- Expression in Escherichia coli of chemically synthesized genes for human insulin.Proceedings of the National Academy of Sciences, 1979
- Evidence for a ninth influenza viral polypeptideVirology, 1978
- Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3α,6α-diphenylglycolurilBiochemistry, 1978
- Antigenic determinants of influenza virus hemagglutinin I. Cyanogen bromide peptides derived from A/MEMPHIS/72 hemagglutinin possess antigenic activityVirology, 1978
- Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone SomatostatinScience, 1977
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970