ABSORPTION OF PHENOTHIAZINE FROM THE RUMEN OF SHEEP, AND ITS ESTIMATION IN BLOOD SERUM

Abstract

Under the conditions described, phenothiazine is shown to be soluble in human and sheep serum. Ultraviolet examination of serum containing phenothiazine indicates that the compound is unchanged by solution in serum. After preliminary experiments with ultraviolet analysis the visual spectra of the free radicals were found to be more suitable for the analysis of sheep serum. A method is described, which makes use of free-radical absorption spectra, for the detection and approximate estimation of phenothiazine in serum when it is present with phenothiazone. The method also allows for the detection of diphenylamine o-sulphoxide. Experiments with lambs which had been dosed with phenothiazine showed that while the peripheral circulation contained leucophenothiazone, the blood draining the rumen contained phenothiazine also.