Surface-Enhanced Raman Analysis of Benzo[A]Pyrene-DNA Adducts on Silver-Coated Cellulose Substrates

Abstract

Benzo[a]pyrene-7,8-diol-9,10 epoxide (BPDE)-DNA adducts have been analyzed by surface-enhanced Raman scattering (SERS) spectrometry. BPDE is the ultimate carcinogenic metabolite of benzo[a]pyrene. The SERS measurements were performed with the use of a silver-coated cellulose material as the SERS-active substrate. The acid hydrolyzed products of BPDE-DNA adducts, DNA, and the BP-tetrols were also analyzed by SERS. The results indicate that SERS has great promise as a tool for detecting BPDE-DNA adducts in biological samples.