SecA protein is directly involved in protein secretion in Escherichia coli
- 2 January 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 242 (2), 431-434
- https://doi.org/10.1016/0014-5793(89)80516-2
Abstract
A high-expression plasmid for the secA gene was constructed. The SecA protein was then overproduced in E. coli and purified. The purified SecA stimulated the in vitro translocation of a model secretory protein into inverted membrane vesicles pretreated with 4 M urea. Membrane vesicles from a secAts mutant exhibited lower translocation activity, which was enhanced by SecA. These results indicate that SecA is directly involved in protein secretion across the cytoplasmic membraneKeywords
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