Cloning and characterization of a gene for a 19kDa fibrinogen‐binding protein from Staphylococcus aureus
- 1 May 1994
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 12 (4), 599-606
- https://doi.org/10.1111/j.1365-2958.1994.tb01046.x
Abstract
Summary: Staphylococcus aureus has been shown to interact specifically with fibrinogen. Three different extracellular fibrinogen‐binding proteins, two of which have coagulase activity, are produced by S. aureus strain Newman. The role of these fibrinogen‐binding proteins during staphylococcal colonization and infection has not yet been fully elucidated. Here we describe the cloning, sequencing and expression of a gene for a 19kDa fibrinogen‐binding protein. This gene, called fib, encodes a 165‐amino‐acid polypeptide, including a 29‐amino‐acid signal sequence. The recombinant protein, which has an estimated molecular mass of 15.9kDa, bound fibrinogen and was recognized by a polyclonal antiserum against the native Fib protein. Homologies between the Fib protein and the fibrinogen‐binding domain of coagulase suggest that amino acids within this domain are involved in the binding to fibrinogen.Keywords
This publication has 45 references indexed in Scilit:
- Interactions of bacteria with extracellular matrix proteinsCell Differentiation and Development, 1990
- Neutrophil bactericidal activity against Staphylococcus aureus adherent on biological surfaces. Surface-bound extracellular matrix proteins activate intracellular killing by oxygen-dependent and -independent mechanisms.JCI Insight, 1990
- The Role of Fibrinogen in Staphylococcal Adherence to Catheters In VitroThe Journal of Infectious Diseases, 1990
- The coagulase of Staphylococcus aureus 8325‐4. Sequence analysis and virulence of site‐specific coagulase‐deficient mutantsMolecular Microbiology, 1990
- Host Factors Selectively Increase Staphylococcal Adherence on Inserted Catheters: A Role for Fibronectin and Fibrinogen or FibrinThe Journal of Infectious Diseases, 1989
- Adherence of staphylococci to intravascular cathetersJournal of Medical Microbiology, 1989
- Species‐specific variation in signal peptide design Implications for protein secretion in foreign hostsFEBS Letters, 1989
- Bacillus subtilis Requires a "Stringent" Shine-Dalgarno Region for Gene ExpressionDNA, 1984
- Isolation, characterization and synthesis of peptides from human fibrinogen that block the staphylococcal clumping reaction and construction of a synthetic clumping particleBiochemistry, 1982
- REGULATORY SEQUENCES INVOLVED IN THE PROMOTION AND TERMINATION OF RNA TRANSCRIPTIONAnnual Review of Genetics, 1979