REPAIR OF 4,5',8-TRIMETHYLPSORALEN PLUS LIGHT-INDUCED DNA DAMAGE IN NORMAL AND FANCONI ANEMIA CELL-LINES
- 15 April 1988
- journal article
- research article
- Vol. 48 (8), 2015-2020
Abstract
In conditions in which solely monoadducts (MA) are induced in DNA, treatment with 4,5'',8-trimethylpsoralen and 405 nm radiation, Fanconi''s anemia cells (FA) appear to be more sensitive than normal human fibroblasts (1 BR/3) to cytotoxicity. The repair of such induced MA is impaired in FA compared to normal cells. When increasing the proportion of DNA interstrand cross-links (CL) over MA using a reirradiation protocol, the differential sensitivity between FA and normal human cells increases. Moreover, for constant number of total adducts or at different ratios of CL over MA, the repair of CL is systematically hampered in FA as compared to normal cells. Incision of CL being progressively diminished by increasing amounts of MA in normal cells (D. Papadopoulo, D. Averbeck, and E. Moustacchi, Photochem. Photobiol., 47:321 -326, 1988), we show here that it is even more so far FA cells.This publication has 6 references indexed in Scilit:
- HIGH LEVELS OF 4,5',8-TRIMETHYLPSORALEN PHOTOINDUCED FURAN-SIDE MONOADDUCTS CAN BLOCK CROSS-LINK REMOVAL IN NORMAL HUMAN CELLSPhotochemistry and Photobiology, 1988
- Response of Human Fibroblasts to Low Dose Rate Gamma IrradiationRadiation Research, 1984
- Response of lymphocytes from Fanconi's anemia patients and their heterozygous relatives to 8-methoxy-psoralene in a cloning survival test systemHuman Genetics, 1983
- Failure of diepoxybutane to enhance sister chromatid exchange levels in Fanconi's anemia patients and heterozygotesHuman Genetics, 1983
- Higher inductions of twin and single sister chromatid exchanges by cross-linking agents in Fanconi's anemia cellsHuman Genetics, 1982
- The fate of 8-methoxypsoralen photoinduced crosslinks in nuclear and mitochondrial yeast DNA: comparison of wild-type and repair-deficient strains.Proceedings of the National Academy of Sciences, 1982