Flow cytometry as a tool for the study of cell kinetics in epidermis.

Abstract

Flow cytometric measurements of the DNA content were performed on a large number of skin biopsies by an automated technique. Expressed as a percentage of all viable cells in the epidermis, the figures for cells in S[DNA synthesis]-phase averaged 1.8% and for G2M [2nd growth phase and mitosis] 0.9%. No significant differences due to sex were found. With age the ratio S/G2M (representing the duration of S to the duration of G2M) increased. Seasonal effects showed higher values for S and G2M in June compared to Nov. and Dec. Small differences dependent on body-site were found, the ratio S/G2M being greater in legs than in arms.