Calcium oscillation associated with reduced protein kinase C activities inras-transformed NIH3T3 cells

Abstract

We show here novel intracellular Ca²⁺ oscillation in ν‐K‐ras‐transformed NIH3T3 cells induced by mitogenic peptide hormones, bradykinin and bombesin, as well as fetal calf serum. Induction of the Ca²⁺ oscillation is strongly correlated with the malignant properties and inversely with PKC activities in vitro and in vivo. These results suggest that the mitogen‐induced Ca²⁺ oscillation is negatively regulated by PKC, which modulates Ca²⁺ influx in ν‐K‐ras‐transformed NIH3T3 cells.