Potential and Bottlenecks of Bioreactors in 3D Cell Culture and Tissue Manufacturing
- 28 August 2009
- journal article
- other
- Published by Wiley in Advanced Materials
- Vol. 21 (32-33), 3352-3367
- https://doi.org/10.1002/adma.200802748
Abstract
Over the last decade, we have witnessed an increased recognition of the importance of 3D culture models to study various aspects of cell physiology and pathology, as well as to engineer implantable tissues. As compared to well-established 2D cell-culture systems, cell/tissue culture within 3D porous biomaterials has introduced new scientific and technical challenges associated with complex transport phenomena, physical forces, and cell–microenvironment interactions. While bioreactor-based 3D model systems have begun to play a crucial role in addressing fundamental scientific questions, numerous hurdles currently impede the most efficient utilization of these systems. We describe how computational modeling and innovative sensor technologies, in conjunction with well-defined and controlled bioreactor-based 3D culture systems, will be key to gain further insight into cell behavior and the complexity of tissue development. These model systems will lay a solid foundation to further develop, optimize, and effectively streamline the essential bioprocesses to safely and reproducibly produce appropriately scaled tissue grafts for clinical studies.Keywords
This publication has 120 references indexed in Scilit:
- Insulin-like Growth Factor-I and Slow, Bi-directional Perfusion Enhance the Formation of Tissue-Engineered Cardiac GraftsTissue Engineering, Part A, 2009
- Cardiac tissue engineering using perfusion bioreactor systemsNature Protocols, 2008
- An in silico bioreactor for simulating laboratory experiments in tissue engineeringBiomedical Microdevices, 2008
- Engineered cartilage generated by nasal chondrocytes is responsive to physical forces resembling joint loadingArthritis & Rheumatism, 2007
- Enabling Sensor Technologies for the Quantitative Evaluation of Engineered TissueAnnals of Biomedical Engineering, 2007
- Effect of reduced oxygen tension and long-term mechanical stimulation on chondrocyte-polymer constructsCell and tissue research, 2007
- Activation of the ERK1/2 Cascade via Pulsatile Interstitial Fluid Flow Promotes Cardiac Tissue AssemblyTissue Engineering, 2007
- The beneficial effect of delayed compressive loading on tissue-engineered cartilage constructs cultured with TGF-β3Osteoarthritis and Cartilage, 2007
- Monitoring of metabolite gradients in tissue-engineered constructsJournal of The Royal Society Interface, 2006
- Mechanobiology of engineered cartilage cultured under a quantified fluid-dynamic environmentBiomechanics and Modeling in Mechanobiology, 2002