The Preparation from Baker's Yeast of Yeast Nucleic Acid Containing a High-Polymer Fraction.

Abstract

Earlier methods were compared. The best method to obtain relatively highly polymerized fractions involves short treatment at low temp. with relatively strong alkali. A specimen with a relatively monodisperse, highly polymerized fraction having a dielectric molecular wt. of approx. 65,000 was obtained by a modification of Johnson and Harkin''s method. The high-polymer fraction is very unstable even when in air-dry condition with a low moisture content. The preparation must be performed rapidly and the investigations made on freshly prepared specimens.