Pulmonary Vascular Changes in the Mouse During Anaphylactic Shock.

Abstract

Direct microscopic observation of the living pulmonary circulation of mice was performed using the quartz rod method of Knisely. Mice were sensitized intra-peritoneally with 0.1 ml of Hemophilus pertussis vaccine and 0.05 ml of horse serum. Challenge doses of horse serum (0.2 ml) were given intravenously 10-20 days later. Pulmonary blood flow was noted before and after the intravenous challenge dose of horse serum. Arteriolar constriction, clumping of red cells and embolization were observed.