The production of glucose by the kidney was studied in eviscerated rabbits, using radioactive glucose as a tracer. The specific activity of the circulating glucose was followed as an indicator of any addition of new glucose from a body source. After 1 injn. of marked glucose and then allowing the animals to become hy-poglycemic, the specific activity of the circulating glucose would be reduced if the kidneys were left intact. The activity was not changed if the kidneys were removed. This indicates that the kidney makes new glucose under these conditions. When the blood sugar was maintained for 8 hrs. at a normal level by injn. of glucose soln. of known radioactivity, the specific activity of the terminal plasma glucose was found to be similar to that of the injected glucose, indicating that no appreciable amt. of new glucose was added by the kidneys. Evidence is presented which supports the view that the kidney in these circumstances may form glucose from fragments of circulating glucose.