Evaluation of New Microparticulate Packings for Aqueous Steric Exclusion Chromatography

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Abstract
Two types of high performance aqueous size steric exclusion chromatography (SEC) columns were recently developed; a rigid spherical silica-based packing containing a new hydrophilic bonded phase (MicroPak TSK Gel Type SW), and an organic-based, semi-rigid gel (MicroPak TSK Gel Type PW). Characteristics of MicroPak TSK SW and PW columns were compared to other commercially available aqueous SEC columns packed with similar supports. Chromatographic performance of prepacked columns containing microparticulate support materials was compared for exclusion separations of water-soluble organic polymers, biopolymers and small water-soluble oligomers. Amino acid probes were used to investigate non-exclusion effects of MicroPak TSK SW and PW columns.