A type II restriction endonuclease with an eight nucleotide specificity fromStreptomyces fimbriatus
Open Access
- 11 June 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (11), 4507-4516
- https://doi.org/10.1093/nar/12.11.4507
Abstract
A new site-specific endonuclease, Sfi I, has been isolated from Streptomyces fimbriatus. This is the first report of a type II restriction endonuclease whose recognition specificity requires eight nucleotides. Sfi I cleaves the sequence, GGCCNNNN/NGGCC, symmetrically to produce a three base, 3′ extension.Keywords
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