Release of platelet constituents by monosodium urate crystals.
Open Access
- 1 November 1977
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 60 (5), 999-1007
- https://doi.org/10.1172/jci108880
Abstract
The release of human platelet constituents by the etiologic agent of gout, the monosodium urate crystal, is described here. In suspensions of washed platelets, response to urate crystals proceeded in two phases: A secretory phase involved the rapid active release of serotonin, ATP, and ADP with little loss of lactic dehydrogenase or beta-glucuronidase. A lytic phase involved the slower loss of all platelet constituents. Both phases were inhibited by iodoacetate plus dinitrophenol, suggesting an energy requirement. In ultrastructural studies, lysis of washed platelets which appeared to contain crystals was seen. Urate crystals were also shown to induce serotonin release and platelet lysis in citrated platelet-rich plasma. Since urate crystals are deposited at a variety of sites, urate crystal-platelet interaction in vivo is a possibility. Such interactions, leading to release of platelet constituents, might contribute to gouty inflammation or to enhanced atherogenesis.This publication has 38 references indexed in Scilit:
- Studies on Platelet Factor-3 AvailabilityBritish Journal of Haematology, 1965
- The Kaolin Clotting Time of Platelet‐Rich Plasma: A Test of Platelet Factor‐3 AvailabilityBritish Journal of Haematology, 1965
- INFLAMMATORY PROCESS IN ACUTE GOUTY ARTHRITIS .I. ACTIVATION OF HAGEMAN FACTOR BY SODIUM URATE CRYSTALS1965
- STUDY OF PLATELET ADHESIVENESS AND AGGREGATION WITH LATEX PARTICLES1965
- Platelet Sequestration in Man. I. Methods*JCI Insight, 1964
- ACTIVATION OF HAGEMAN FACTOR BY SOLUTIONS OF ELLAGIC ACID.1964
- A comparison of the duration of local anti-inflammatory effect of several adrenocorticosteroid esters--a bioassay technique.1963
- BLOOD COAGULATION AND PLATELET ECONOMY IN SUBJECTS WITH PRIMARY GOUT1963
- The release of histamine and 5‐hydroxytryptamine (serotonin) from platelets by antigen‐antibody reactions (in vitro)The Journal of Physiology, 1955
- SPECIFIC VASCULAR CHANGES IN GOUTJAMA, 1954