Intrauterine and Intravaginal Insemination with Frozen Canine Semen Using an Extender Consisting of Orvus ES Paste-Supplemented Egg Yolk Tris-Fructose Citrate
- 1 January 2000
- journal article
- Published by Japanese Society of Veterinary Science in The Journal of Veterinary Medical Science
- Vol. 62 (6), 603-606
- https://doi.org/10.1292/jvms.62.603
Abstract
Our previous report indicated that addition of Orvus ES Paste (OEP) to the extender of frozen canine semen protected acrosomes and maintained sperm motility after thawing. In this study, artificial insemination (AI) using the frozen semen was carried out. The frozen semen was prepared using egg yolk Tris-fructose citrate, and the final concentrations of glycerol and OEP were 7% (v/v) and 0.75% (v/v), respectively. AI was performed during the optimal mating period predicted from the peripheral plasma progesterone level. In intrauterine insemination (IUI), the bitches were laparotomized and 1 x 10(8) spermatozoa were infused into one of the uterine horns. In insemination of non-OEP supplemented semen, 3 x 10(8) spermatozoa were inseminated. In intravaginal insemination (IVI), 10-40 x 10(8) spermatozoa were inseminated. Conception was obtained in nine of 10 bitches (90.0%) that underwent IUI. The number of newborns was from 1 to 7 (mean 3.6 +/- 0.9). The mean ratio of the number of puppies to the number of ovulations in the inseminated uterine horn was 71.8%. The number of puppies did not exceed the number of ovulation in the inseminated uterine horn. Conception using non-OEP supplemented frozen semen was unsuccessful in all four bitches. In IVI, conception was not obtained in any of the six bitches that received insemination of 10 x 10(8) or 40 x 10(8) spermatozoa, but two of three bitches that received insemination of 20 x 10(8) spermatozoa were fertilized. It was shown that a high conception rate can be obtained by IUI using OEP-supplemented frozen canine semen. Developmenmt of a non-surgical method of IUI and a method of freezing canine sperm applicable to IVI is necessary.Keywords
This publication has 15 references indexed in Scilit:
- Effect of Addition of Orvus ES Paste to Frozen Canine Semen Extender on Sperm Acrosomes.The Journal of Veterinary Medical Science, 2000
- Effects of Orvus ES Paste on Canine Spermatozoal Longevity after Freezing and ThawingThe Journal of Veterinary Medical Science, 2000
- Plasma LH and Progesterone Levels before and after Ovulation and Observation of Ovarian Follicles by Ultrasonographic Diagnosis System in Dogs.The Journal of Veterinary Medical Science, 2000
- Fertility after vaginal or uterine deposition of dog semen frozen in a tris extender with or without equex STM pasteTheriogenology, 1999
- Comparisons of intravaginal and intrauterine insemination of bitches with fresh or frozen semenVeterinary Record, 1996
- Relationship between the number of sperms and the rate of implantation in bitches inseminated into unilateral uterine horn.The Japanese Journal of Veterinary Science, 1989
- Influence of extender, cryoperservative and seminal processing procedures on postthaw motility of canine spermatozoa frozen in strawsTheriogenology, 1989
- Transport of spermatozoa in the reproductive tract of the bitch: Observations through uterine fistula.The Japanese Journal of Veterinary Science, 1989
- Artificial insemination with fresh semen in beagle bitches.The Japanese Journal of Veterinary Science, 1988
- Capacitation, acrosome reaction, and egg penetration by canine spermatozoa in a simple defined mediumGamete Research, 1978