1,25-dihydroxyvitamin D3 potentiates fluoride-stimulated collagen type I production in cultures of human bone marrow stromal osteoblast-like cells

Abstract

In this study we tested the effects of sodium fluoride (NaF) in serum‐free cultures of human marrow stromal osteoblast‐like [hMS(OB)] cells. NaF (10⁻⁵ M) stimulated hMS(OB) cell proliferation up to 220% of control cultures. NaF alone did not increase type I collagen production, but in the presence of 1,25‐dihydroxyvitamin D₃ [1,25‐(OH)₂D₃] (10⁻⁹ M), NaF enhanced type I collagen production in a dose‐dependent way to 300% of 1,25‐(OH)₂D₃‐treated control cultures. The production of alkaline phosphatase (ALP) and osteocalcin (bone gla protein, BGP) was also enhanced in the presence of 1,25‐(OH)₂D₃ to 170 and 200%, respectively, of 1,25‐(OH)₂D₃‐treated controls. Our results suggest that 1,25‐(OH)₂D₃ potentiates fluoride‐mediated anabolism in hMS(OB) cell cultures and suggest that osteoblast precursors in bone marrow are targets for fluoride action.