CHEMICAL IDENTIFICATION OF IODINE-CONTAINING COMPOUNDS IN HUMAN SERUM1

Abstract

Studies have been made on the extraction of iodinated compounds from human serum, their chromato-graphic separation on filter paper, and their detection by a cerie sulfate-arsenious acid reagent, followed by staining with aniline in acetone and neutralizing with sodium bicarbonate. Chloride simulated iodine compounds on chromatograms. Thiouracil, thiouracil disulfide and propylthiouracil were found to reduce cerie ion catalytically and when run in certain solvent systems moved to positions which could cause confusion with the iodotyrosines or iodothyronines. Only thyroxine and iodide were regularly identified in normal human serum, but small amounts of unknown positive-reacting compounds were found among patients with simple goiter.