shRNA libraries and their use in cancer genetics
- 23 August 2006
- journal article
- review article
- Published by Springer Nature in Nature Methods
- Vol. 3 (9), 701-706
- https://doi.org/10.1038/nmeth921
Abstract
RNA interference was originally described as a powerful tool to inhibit gene expression in model organisms. Until recently, loss-of-function genetic screens in mammalian cells were hampered by a lack of suitable tools that can be used in a high-throughput format. Here we discuss the construction of short-hairpin RNA (shRNA) vector libraries, in particular those generated at the Netherlands Cancer Institute (NKI), and their application in mammalian cancer genetics. We describe their virtues and limitations, as well as different options for screening such libraries.Keywords
This publication has 27 references indexed in Scilit:
- A loss-of-function RNA interference screen for molecular targets in cancerNature, 2006
- Second-generation shRNA libraries covering the mouse and human genomesNature Genetics, 2005
- A Genetic Screen for Candidate Tumor Suppressors Identifies RESTCell, 2005
- The Deubiquitinating Enzyme USP1 Regulates the Fanconi Anemia PathwayMolecular Cell, 2005
- A resource for large-scale RNA-interference-based screens in mammalsNature, 2004
- New tools for functional mammalian cancer geneticsNature Reviews Cancer, 2003
- Functional siRNAs and miRNAs Exhibit Strand BiasCell, 2003
- Asymmetry in the Assembly of the RNAi Enzyme ComplexCell, 2003
- Arrayed adenoviral expression libraries for functional screeningNature Biotechnology, 2002
- A System for Stable Expression of Short Interfering RNAs in Mammalian CellsScience, 2002