Chromosome Changes Associated With the Progression of Cell Lines From Preneoplastic to Tumorigenic Phenotype During Transformation of Mouse Salivary Gland Epithelium In Vitro
Two mouse salivary gland epithelial cell lines, CSG 211 and CSG 205/281, isolated during carcinogen-induced neoplastic transformation in vitro, were analyzed cytogenetically before and after they acquired the ability to produce carcinomas in syngeneic animals. With the use of Giemsa banding techniques, chromosome changes were identified that were associated with the transition from a preneoplastic to a fully transformed (tumorigenic) phenotype during serial passage in vitro. Results were compared with those from a third cell line of similar origin, CSG 225, which was tumorigenic at the earliest passagetested. These cell lines were found to be subtetraploid, which confirms previous data, and the tumorigenic lines showed consistent losses of copies of chromosomes 1, 4, 7, 9, and 14. Compared with their preneoplastic counterparts, the loss of no single chromosome seemsto be sufficient to generate the tumorigenic phenotype, but the loss of a combination of some or all of these chromosomes appears to be important in the phenotypic transition. In CSG 211 the loss of chromosome 7 is probably more important in this respect than loss of the other chromosomes listed. The karyotype of this cell line undergoes major structural rearrangement, which suggests that loss of specific regions of chromosomes 1 and 9 is also important.