METABOLIC AND CHEMICAL PROPERTIES OF BASIC PROTEINS ISOLATED FROM NUCLEI OF RAT LIVER AND THYMUS GLAND

Abstract

The effects of alkylating agents and disulphides on the thiol-containing proteins of nuclei from rat thymus and liver were studied. Three protein fractions were examined histones extracted with 50mM- and 250mM-hydrochloric acid and the residual protein. None of the reagents selectively reacted with any one of the protein fractions. Amino acid uptake in vitro into the histones of nuclei from rat thymus was analysed by preparative electrophoresis of the proteins extracted with 50mM- and 250mM-hydrochloric acid. After 1 hr. at 37[degree] the greater incorporation was into the proteins extracted with 50mM-50mM-hydrochloric acid. Preparative electrophoresis was used to study the relative thiol contents of the proteins of the 50mM-hydrochloric acid extract from thymus nuclei by labelling the histones in vitro with C14-labelled N-ethylmaleimide. The capacity of the proteins extracted from rat thymus with 50mM- and 250mM-hydrochloric acid, and of the components from these extracts separated by preparative electrophoresis, to combine with DNA and to depress DNA-dependent RNA synthesis was studied. The histones extracted with 50mM-hydrochloric acid were more lysine-rich than those extracted with 250mM-hydro-chloric acid. Wide variations were found in the abilities of the separated components to depress RNA synthesis.