Repetitive DNA sequences cotranscribed with developmentally regulated Dictyostelium discoideum mRNAs.
- 1 September 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (9), 5386-5390
- https://doi.org/10.1073/pnas.78.9.5386
Abstract
A clone of D. discoideum genomic DNA, pB41-6, was isolated that contains sequences that are reiterated and interspersed in the genome. The expression of these reiterated sequences is developmentally regulated, and they appear to be linked only to developmentally regulated mRNA. Clone pB41-6 was used to isolate a set of complementary cDNA clones. Analysis of 2 selected clones indicates that they encode developmentally regulated mRNA species and, by means of the repetitive elements they contain, hybridize to many developmentally regulated mRNA. In addition, 2 families of mRNA species carrying these repetitive sequences were identified: one is induced at 5.5 h of development and the other is induced between 5.5 and 15 h of development. Since most of the mRNA species that have sequences complementary to clone pB41-6 accumulate in a coordinated fashion during development, these sequences may play an important regulatory role in the developmental program of Dictyostelium.Keywords
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