Substrate utilization for lactate and energy production by heat‐shocked L929 cells

Abstract

The hypothesis that heat shock protein (HSP) induction depends on inhibition of respiration was tested by examining the effects of heat shock on tricarboxylic acid (TCA) cycle function. In control L929 cell cultures, glucose and exogenous pyruvate were converted primarily to lactate, and glutamine was extensively oxidized, accounting for more than one-half of the calculated ATP production. During heat shock at 42°C, lactate production from all of the labeled substrates and total unlabeled lactate production increased significantly while oxygen consumption increased slightly. TCA cycle oxidation of pyruvate decreased during this period while that of glutamine increased. Uncoupling of oxidative phosphorylation caused large increases in oxygen consumption at both 37°C and 42°C, indicating that the capacity of the respiratory chain is not exceeded during heat shock. The net effect of these alterations in substrate utilization were decreased ATP generation and increased NADH utilization. Both ¹⁴CO₂ and lactate production declined during the 24-h period after cultures were returned to 37°C. On the basis of these data, we conclude that while inhibition of respiration plays no apparent role other metabolic consequences of heat shock related to energy metabolism may be involved in HSP induction.