The Separation of Orthopadics from the Cell Envelope in Rhodopseudophas Spheroiders

Abstract

When subcellular particles from. Rhodopseudophas spheroices wore laced on sucrose density gradients, the separation of chromatophores from the cell envelope was markedly affected by the presence of ionic species. In gradients that contained Tris buffer plus 0.01’ magnesium, chromatophores were distributed nearly equally between an upper and a lower pigmented band. About half of the chronatopnores were release from the lower band when magnesium was excluded from the gradients. exclusion of both Tris and magnesium resulted in a quantitative separation of chromatophores (upper band) from the cell envelope (lower ‘and). Thus, the photosynthotic apparatus in Rps. spheroides resides on a membrane system separable from the cell wall-cell membrane complex.