Abstract
Stimulation of the vulva and cervix by massage caused an increase in intramammary pressure in 88% of 99 trials on 16 cows for an avg. increase of 8.0 cm. of water. In 15 natural matings, 80% gave a positive response for an avg. increase of 4.5 cm. of water. Blood taken before massage of the vulva and cervix had no effect, but blood taken after stimulation caused, in each of 3 trials, contraction of excised, perfused cow uteri similar to that caused by oxytocin. Stimulation of the reproductive organs of the cow probably causes the release of oxytocin.