Isolation and biochemical characterization of a zona pellucida‐binding glycoprotein of boar spermatozoa
- 11 March 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 280 (1), 183-186
- https://doi.org/10.1016/0014-5793(91)80233-s
Abstract
Lectin-like molecules on the sperm surface are implicated in the process of gamete recognition and adhesion. We have isolated and biochemically characterized a 15 kDa glycoprotein from ejaculated boar sperm which possess zona pellucida-binding- and haemagglutinating-activity. The zona/ 15 kDa protein interaction is inhibited by fucoldan, suggesting that the glycoprotein is one of the sperm components which participate in the initial gamete interaction. N-Terminal sequence analysis of the isolated 15 kDa glycoprotein showed that it may belong to the same sperm/egg recognition-mediating protein family as the sea urchin sperm protein binding.Keywords
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