Abstract
Treatment of human diploid fibroblasts with fibroblast interferon for 8 h inhibited replication of vesicular stomatitis virus. When the total cell protein of cells treated with interferon for 8 h was compared to the total cell protein of untreated cells by 2-dimensional gel electrophoresis, the interferon-treated cells were found to contain 4 proteins not found in untreated cells. Addition of actinomycin D to the cells concurrently with interferon inhibited the synthesis of the 4 proteins. These proteins are apparently induced by interferon and they may be involved in the inhibition of virus replication.