Isolation and regional mapping of random X sequences from distal human X chromosome

Abstract

Chromosome-mediated gene transfer (CMGT) lines were shown to be convenient donors of genomic sequences from specific regions of the genome adjacent to selectable markers. Two libraries were prepared from CMGT lines carrying sequences spanning the long arm of the human X chromosome from HPRT(Xq26) to G6PD(Xq28). A series of 22 CMGT lines sharing the same selectable marker (HPRT)were used in conjunction with five standard translocation hybrids to provide fine-resolution regional mapping of the nonrepetitive X specific probes isolated from the libraries. The order of three human recombinant sequences with respect to known X-linked markers is: PGK(Xq13), 05-02 (DXS78); HPRT(Xq26), 07-03 (DXS79);surface antigen S11(Xq27), 07–14 (DXS80); and G6PD (Xq28).