Farbstoffbindungs-Studien an Mitochondrien II

Abstract

The liver mitochondria fraction from rats after the addition of stains in vitro contains substantially more trypan blue than after storage in life. In comparison with the star cells of Kupffer, the liver capillaries of the rat or the membrane of the liver parenchyma cells are quite impermeable to trypan blue and lithium carmine and, accordingly, also, to dye-containing serum albumin and globulins. Of the trypan blue stored in vivo by liver mitochondria, 20 to 45% is desorbed in vitro by different sera. Conversely, 60 to 70% of the stain, under our experimental conditions, is absorbed in vitro from dye-containing sera. Even 15 min. after intravenous injection of trypan blue more than 50% of the dye is no longer evident in the serum. The liver mitochondria, in this time, have absorbed about 40% of the amount of dye stored in them in 24 hours.