Separation of Cell Components in the Zonal Ultracentrifuge

Abstract

Zonal separation of particles in density gradients contained in hollow or tubeless rotors has been explored with the aim of developing a preparative counterpart of the analytical ultracentrifuge. A rotor with a capacity of 1625 milliliters has been tested up to 22,500 revolutions per minute, with sucrose density gradients used. Excellent separation of subcellular particles has been achieved as well as partial separation of the albumin and globulin peaks of serum.