Characterization of monoclonal antibodies against human protein C specific for the calcium ion‐induced conformation or for the activation peptide region
- 21 October 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 191 (1), 75-81
- https://doi.org/10.1016/0014-5793(85)80997-2
Abstract
Three monoclonal antibodies have been produced that are specific for the activation peptide region m human protein C. These antibodies inhibited the activation of protein C by thrombin and by the thrombin-thrombomodulin complex. A fourth monoclonal antibody specifically recognized the Ca2+-stabilized conformation in protein C. This antibody bound both intact protein C and protein C from which the γ-carboxy-glutamic acid-containing region had been removed by limited proteolysis. These results indicate that this antibody recognizes the conformation in protein C stabilized by Ca2+ bound to the single binding site that is independent of γ-carboxyglutamic acid.Keywords
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