SPECIFIC RADIOIMMUNOASSAY OF CYCLO (HIS-PRO), A BIOLOGICALLY ACTIVE METABOLITE OF THYROTROPIN-RELEASING HORMONE
- 1 May 1981
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 108 (5), 1995-1997
- https://doi.org/10.1210/endo-108-5-1995
Abstract
We have developed a radioimmunoassay that can detect 10 pg cyclo (His-Pro) in rat brain perchloric acid extracts. The normal rat brain concentration of cyclo (His-Pro) ranged from 35–61 pmole/brain. The elution profiles of cyclo (His-Pro)-like immunoreactivity and radioactive cyclo (His-Pro) following gel filtration and ion-exchange chromatography were similar.Keywords
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