The Influence of Aliphatic Alcohols on Leaf Senescence

Abstract

Because of the effects of ethanol used as a solvent in other experiments, the action of aliphatic alcohols on leaf senescence in the dark was studied systematically in oat seedlings (Avena sativa cv. Victory). These compounds both maintain chlorophyll and prevent proteolysis in the dark, much as do the cytokinins and other senescence-delaying substances. The activity of the straight-chain alcohols increases in a log-linear fashion with increasing chain length up to 1-octanol. Introduction of a branch in the chain of a 2nd OH group greatly decreases, or in some cases annuls, the antisenescence activity. In all cases, the action on senescence is closely (although not always exactly) paralleled by opening of the stomata. Abscisic acid and exposure to high concentrations of osmoticum, both of which close the stomata, antagonize the action of the alcohols. Some interactions with other agents are noted. The effects are compared with reported effects on seed germination, on hemolysis and animal membranes, and especially on permeability to K+ ions, and a tentative basis for the mechanism of action is advanced.