The molecular mechanism of virus induction
- 1 January 1976
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 148 (2), 131-138
- https://doi.org/10.1007/bf00268376
Abstract
The basic conditions for use of a new biochemical assay of bacteriophage induction are described. This method is based on the read-through transcription of the tryptophan operon integrated into an “early” transcribed region of the bacteriophage ϕ80 or λ genome. Inactivation of repressor molecules was assayed by measuring, in the presence of tryptophan, anthranilate synthetase activity in an Escherichia coli (trpE −) ϕ80 or λ lysogen infected with ϕ80ptrp or λptrp, respectively, and treated with mitomycin C or UV irradiation. This method provides a sensitive and easy means to analyze the induction process.This publication has 23 references indexed in Scilit:
- Regulation of the tryptophan operon of Escherichia coli integrated into the phage ϕ80 genomeMolecular Genetics and Genomics, 1974
- Inactivation of DNA-binding activity of repressor in extracts of λ-lysogen treated with mitomycin CMolecular Genetics and Genomics, 1973
- Prophage excision: Specific gene amplification of excision-impaired lysogen after inductionVirology, 1973
- Integration and excision of ∅80pt prophage in Escherichia coliVirology, 1971
- Ribonucleic acid-permeable mutant of Escherichia coliJournal of Molecular Biology, 1971
- Isolation and characterization of non-defective transducing elements of bacteriophage φ80Virology, 1967
- Effect of ultraviolet irradiation on bacteriophage lambda immunityJournal of Molecular Biology, 1967
- Transduction studies on the role of a rec+ gene in the ultraviolet induction of prophage lambdaJournal of Molecular Biology, 1967
- DNA replication and messenger RNA production after induction of wild-type λ bacteriophage and λ mutantsJournal of Molecular Biology, 1966
- Characteristics of the transducing elements of bacteriophage φ80Virology, 1964