Temporal programming of chloroplast and cytoplasmic ribosomal RNA transcription in the synchronous cell cycle of Chlamydomonas reinhardtii.
Open Access
- 1 February 1977
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 72 (2), 470-481
- https://doi.org/10.1083/jcb.72.2.470
Abstract
Approximately 90% of the Chlamydomonas reinhardtii chloroplast and cytoplasmic rRNAs was transcribed in the nuclear G1 phase, which occurred during the light period under an alternating light-dark synchronization regime of 12 h each. The remaining 10% of chloroplast and cytoplasmic rRNAs was transcribed from its respective DNAs in the dark period, in the midst of an apparent turnover of a transcription appeared to be prokaryotic in sophistication. The transcription was not interrupted during chloroplast DNA synthesis which occurred during the light period. However, transcription of the nuclear DNA was repressed severely during the nuclear S phase in the dark period. The patterns of incorporation of 32P into chloroplast and cytoplasmic rRNA species in the cell cycle were similar to those of the actual rRNA synthesis as measured optically. However, the quantity of 32P incorporation per unit amount of rRNA synthesized varied considerably during the cell cycle, increasing in all rRNA's during the dark period. 32P incorporation data obtained from continuous and pulse 32P-labeling experiments also revealed a turnover of a small amount of both cytoplasmic and chloroplast rRNAs at the end of the S phase. The 32P incorporation into cytoplasmic and chloroplast rRNAs was well matched temporally with the 32P incorporation into their corresponding ribosomes, indicating that the newly synthesized rRNA molecules are utilized without delay throughout the cell cycle in the assembly of ribosomes.This publication has 28 references indexed in Scilit:
- Synthesis and maturation of chloroplast and cytoplasmic ribosomal RNA in Chlamydomonas reinhardiBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
- Rates of synthesis of ribosomal protein and total ribonucleic acid through the cell cycle of the fission yeast Schizosaccharomyces pombeExperimental Cell Research, 1973
- Attachment of Chloroplast Polysomes to Thylakoid Membranes in Chlamydomonas reinhardtiiProceedings of the National Academy of Sciences, 1973
- Changes in cytoplasmic and chloroplast ribosomal ribonucleic acid during the cell cycle of Chlyamydomonas reinhardtiiArchives of Biochemistry and Biophysics, 1973
- Temperature control of initiation of protein synthesis in Escherichia coliJournal of Molecular Biology, 1971
- Denaturation and the renaturation kinetics of chloroplast DNA from Chlamydomonas reinhardiJournal of Molecular Biology, 1971
- The uptake of bases and their incorporation into RNA during the cell cycle of Schizosaccharomyces pombe in normal growth and after a step-downExperimental Cell Research, 1969
- Size Fractionation of Exponentially Growing Escherichia coliNature, 1967
- The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresisBiochemical Journal, 1967
- Synthesis of Ribosomal RNA in Synchronized Hela CellsNature, 1965