In vitro synthesis of the first component of complement by guinea pig small intestine.

Abstract

Evidence is presented for the in vitro synthesis of C[image] [complement] 1 by guinea pig small intestine and for the in vitro incorporation of C14-lysine into hemolytically active C[image]2. Synthesis of C[image]1 by spleen, liver, kidney, lung, testes, esophagus, stomach, cecum, or colon could not be demonstrated. Actinomycin D and puromycin inhibit C[image]1 synthesis by guinea pig small bowel and inhibition can be reversed by returning the tissue to media lacking inhibitors of protein synthesis. In an enriched medium, there is an apparent enhancement of C[image]1 synthesis in the presence of puromycin.