Chemiluminescence enzyme immunoassay of 17.ALPHA.-hydroxyprogesterone using glucose oxidase and bis(2,4,6-trichlorophenyl) oxalate - fluorescent dye system.

Abstract

A highly sensitive enzyme immunoassay of 17α-hydroxyprogesterone has been developed. Glucose oxidase was used as a label enzyme and the double antibody solid phase method was adopted to separate the bound and free fractions after the immune reaction. In the assay of enzyme activity, hydrogen peroxide generated from glucose added as substrate was measured by the chemiluminescence reaction using bis (2, 4, 6-trichlorophenyl) oxalate and 8-anilinonaphthalene-1-sulfonic acid. The detection limit of this method is 0.5 pg/tube. The method is applicable to mass screening for congenital adrenal hyperplasia in neonates.