The distal nephron of rat kidney: a target site for glucagon.
- 1 June 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (6), 3422-3424
- https://doi.org/10.1073/pnas.77.6.3422
Abstract
Glucagon-sensitive adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] activity was measured in 9 different portions of the rat nephron. Each sample contained a single piece of tubule isolated by microdissection from collagenase-treated kidney tissue. Compared to basal activity, 1 .mu.M porcine glucagon stimulated adenylate cyclase 60-fold in the medullary portion and 40-fold in the cortical portion of the thick ascending limb, 23-fold in the early distal convoluted tubule, 11-fold in the cortical collecting tubule, and 8-fold in the medullary collecting tubule. No stimulation was observed in proximal tubules and thin segments of the loop of Henle. Half-maximal stimulations were obtained with .apprx. 10 nM glucagon in the responsive nephron portions.This publication has 11 references indexed in Scilit:
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