The SalGI restriction endonuclease. Purification and properties
- 1 April 1982
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 203 (1), 77-84
- https://doi.org/10.1042/bj2030077
Abstract
The type II restriction endonuclease SalGI has been purified to near homogeneity. At least 80% of the protein remaining after the final stage of the preparation is SalGI restriction endonuclease; no contaminating nucleases remain detectable. The principal form of the protein under both native and denaturing conditions is a monomer of Mr about 29000. The optimal conditions for both enzyme stability and enzyme activity have been determined.This publication has 25 references indexed in Scilit:
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