Nitrogen Fixation by Symbiotic and Free-Living Spirochetes
- 29 June 2001
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 292 (5526), 2495-2498
- https://doi.org/10.1126/science.1060281
Abstract
Spirochetes from termite hindguts and freshwater sediments possessed homologs of a nitrogenase gene (nifH) and exhibited nitrogenase activity, a previously unrecognized metabolic capability in spirochetes. Fixation of 15-dinitrogen was demonstrated with termite gut Treponema ZAS-9 and free-livingSpirochaeta aurantia. Homologs of nifH were also present in human oral and bovine ruminal treponemes. Results implicate spirochetes in the nitrogen nutrition of termites, whose food is typically low in nitrogen, and in global nitrogen cycling. These results also proffer spirochetes as a likely origin of certainnifHs observed in termite guts and other environments that were not previously attributable to known microbes.Keywords
This publication has 28 references indexed in Scilit:
- Phylogenetic diversity of termite gut spirochaetesEnvironmental Microbiology, 1999
- Acetogenesis from H 2 Plus CO 2 by Spirochetes from Termite GutsScience, 1999
- The gut microflora of Reticulitermes flavipes, its relation to oxygen, and evidence for oxygen-dependent acetogenesis by the most abundant Enterococcus sp.FEMS Microbiology Ecology, 1997
- Quartet Puzzling: A Quartet Maximum-Likelihood Method for Reconstructing Tree TopologiesMolecular Biology and Evolution, 1996
- Xylophagous Termites Depending on Atmospheric NitrogenThe Science of Nature, 1994
- Hydrogen Inhibition of Nitrogen Reduction by Nitrogenase in Isolated Soybean Nodule BacteroidsPlant Physiology, 1989
- Ethane formation from acetylene as a potential test for vanadium nitrogenase in vivoNature, 1987
- Comparison of Kjeldahl and combustion methods for measurement of nitrogen isotope ratios in organic matterAnalytical Chemistry, 1984
- Inhibition of nitrogenase-catalyzed ammonia formation by hydrogenBiochemistry, 1983
- Dependence of the higher termite, Nasutitermes exitiosus and the lower termite, Coptotermes lacteus on their gut floraJournal of Insect Physiology, 1978