Chromosomal transformation of Escherichia coli recD strains with linearized plasmids
- 1 May 1989
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 171 (5), 2609-2613
- https://doi.org/10.1128/jb.171.5.2609-2613.1989
Abstract
Wild-type Escherichia coli are resistant to genetic transformation by purified linear DNA, probably in part because of exonuclease activity. We demonstrate that E. coli containing a recD mutation could be easily transformed by linearized plasmids containing a selectable marker. The marker was transferred to the chromosome by homologous recombination, whereas plasmid markers not in the region of homology were lost.This publication has 53 references indexed in Scilit:
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