Ordering of six polymorphic dna markers important in the delineation of 3p deletions in neoplasia

Abstract

Using fluorescence in situ hybridisation (FISH) the chromosomal location and relative order of six human chromosome 3 probes has been determined. The sensitivity of the technique has enabled the relative mapping of probes carrying inserts as small as 500 basepairs (bp), thus allowing the following proximal–distal probe order to be proposed: D3S30 (3p13–14), D3S4 (3p13–14), D3S2 (distal 3p14), D3S32 (3p21), D3S48E (3p21–23), and D3S11 (3p22–23). These data combined with the deletion mapping data of other researchers raise the possibility that the loss of more than one region of the short arm of chromosome 3 may be important in the development of small cell lung cancer.