Synthesis of Phosphatidyl-dCMP in Human Lymphocytes and Its Enhancement by Chlorpromazine
- 1 May 1981
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 116 (2), 249-253
- https://doi.org/10.1111/j.1432-1033.1981.tb05326.x
Abstract
When phytohemagglutinin-stimulated lymphocytes are labeled with [3H]deoxycytidine (dCyd), a compound soluble in organic solvents is formed. Several analytical procedures such as TLC and alkaline hydrolysis of the product obtained after labeling of stimulated lymphocytes with [14C]glycerol and [3H]dCyd suggest that the product synthesized is phosphatidyl-dCMP. This compound is synthesized in much smaller amounts than when permeabilized lymphocytes and [3H] dCTP as a precursor are used. The synthesis of phosphatidyl-dCMP is highly enhanced by chlorpromazine, phosphatidic acid and arachidonic acid; addition of inositol diminishes the amount of detected phosphatidyl-dCMP. This compound may be a precursor of phosphatidylinositol.This publication has 17 references indexed in Scilit:
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