Isolation and structural characterization of novel sialylated oligosaccharide‐alditols from respiratory‐mucus glycoproteins of a patient suffering from bronchiectasis

Abstract

The carbohydrate chains of the respiratory‐mucus glycoproteins of a patient (blood group O) suffering from bronchiectasis due to Kartagener's syndrome, were released by alkaline borohydride treatment of a pronase digest. The structures of 82 neutral and low‐molecular‐mass sialylated oligosaccharides have been described previously [van Kuik A., de Waard P., Vliegenthart J. F. G., Klein A., Carnoy C., Lamblin G. Roussel P. (1991) Eur. J. Biochem. 198, 169–182]. In the present work, medium‐size sialylated oligosaccharides were obtained after ion‐exchange chromatography and were subsequently separated into 36 fractions utilizing gel filtration, HPLC on normal‐phase alkylaminebonded silica and reverse‐phase HPLC. From these fractions, the following six sialylated hepta‐ and octa‐saccharide‐alditols have been characterized by employing 500‐MHz ¹H‐NMR spectroscopy, in conjunction with fast‐atom‐bombardment mass spectroscopy and methylation analysis.