A stereochemical investigation of phosphoryl transfer catalysed by phosphoglucomutase by the use of α-d-glucose 1-[(S)-16O,17O,18O]phosphate
- 1 December 1981
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 199 (3), 693-698
- https://doi.org/10.1042/bj1990693
Abstract
Rabbit muscle phosphoglucomutase converts alpha-D-glucose 1-[(S)-16O,17O,18O]phosphate into D-glucose 6-[16O,17O,18O]phosphate, which is shown by 31P n.m.r. spectroscopy, after cyclization and methylation, to have the (S)-configuration at the phosphorus atom. Since phosphoglucomutase is known to catalyse phosphoryl transfer by way of a phospho-enzyme intermediate, and since individual phosphoryl-transfer steps appear in general to occur with inversion of configuration, this observation is most simply interpreted in terms of a double-displacement mechanism with two phosphoryl-transfer steps.This publication has 8 references indexed in Scilit:
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