Effect of Carbon Tetrachloride on Distribution and Disappearance of Labeled Insulin in Rabbit Serum.

Abstract

The disappearance rate and distribution of I131 labeled insulin in the serum of white rabbits was studied under various degrees of injury with CCl4 and after bilateral nephrectomy. Radioactivity was determined in the protein bound phase. Disappearance of labeled material from the serum could be expressed by a 1st order relationship. Disappearance rate depended most on the presence of intact kidneys and was relatively independent of CCl4 damage to the liver. The theoretical value for serum concentration of labeled material at time O was increased by severe CCl4 damage to the liver but was relatively independent of the status of kidney function. Lower glucose values in the blood, both fasting and after insulin, were found in the CCl4 injured animals.