Straightforward Procedure for Estimating the Spreading Factor in SEC

Abstract

This contribution compiles SEC plate-height data obtained with various polymer standards and with a low-molecular probe. The latter value is easily measured and commonly given as a test of the apparatus used. Plate-height values h from the low and high-molecular range can be approximated by a straight line when plotted logarithmically Knowledge of the slope factor B would enable plate-height data in the Mgh-molecular range to be estimated on the basis of the reliable value from a low-molecular probe. The variance σ² and the spreading factor 1/(2 σ²) can easily be derived from the plate height.