Use of Antisera in the Isolation of Human Specific Conjugates of Haloperidol
- 1 January 1989
- journal article
- research article
- Published by Taylor & Francis in Xenobiotica
- Vol. 19 (7), 781-793
- https://doi.org/10.3109/00498258909042315
Abstract
1. Three conjugated metabolites of haloperidol were isolated from urine of patients on haloperidol and purified by h.p.l.c. with immunological detection, using three types of anti-haloperidol antisera. 2. Structures of the metabolites were: a sulphate conjugate of the 2-hydroxylated 4-fluorophenyl ring of reduced haloperidol (MH-1), a glucuronide conjugate at the same position as MH-1 (MH-2), and a glucuronide conjugate of the hydroxy group of haloperidol (MH-3). 3. MH-3 was the main urinary metabolite in volunteers receiving haloperidol, who excreted 18% of the dose in the 24h urine as MH-3, while other conjugates were less than 1%. MH-3 could not be hydrolysed with β-glucuronidase, due to steric hindrance. 4. Immunological detection of conjugated metabolites is very useful in metabolic studies in humans because of its sensitivity and specificity.This publication has 10 references indexed in Scilit:
- Simultaneous determination of haloperidol and its reduced metabolite in serum and plasma by isocratic liquid chromatography with electrochemical detection.Clinical Chemistry, 1983
- BIOTRANSFORMATION OF BROMPERIDOL IN RAT, DOG, AND MAN1983
- A novel conjugate as a major metabolite of bromperidol in manJournal of Pharmacy and Pharmacology, 1982
- Radioimmunoassay of haloperidol in human serum: Correlation of serum haloperidol with serum prolactinLife Sciences, 1981
- Serum Haloperidol Determinations in Psychiatric PatientsArchives of General Psychiatry, 1980
- Radioimmunoassay of bromperidol and haloperidol in human and canine plasmaEuropean Journal of Drug Metabolism and Pharmacokinetics, 1980
- Determination of haloperidol in human serum by radioimmunoassay.Journal of Pharmacobio-Dynamics, 1980
- A gas chromatographic method for determining haloperidolNaunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie, 1974
- Distribution excretion and metabolism of neuroleptics of the butyrophenone type: Part II. Distribution, excretion and metabolism of haloperidol in Sprague-Dawley ratsEuropean Journal of Pharmacology, 1967