METAPLASIA OF SMOOTH-MUSCLE CELLS INTO JUXTAGLOMERULAR CELLS IN JUXTAGLOMERULAR APPARATUS, ARTERIES, AND ARTERIOLES OF ISCHEMIC (ENDOCRINE) KIDNEY - ULTRASTRUCTURAL-CYTOCHEMICAL AND AUTORADIOGRAPHIC STUDY

Abstract

Partial ligation of the aorta between the renal arteries induces marked atrophy of the cortical tubules of the left (endocrine) [rat] kidney with a remarkable increase in the number and granularity of hypersecretory juxtaglomerular cells (JGC), which are found not only at the glomerular pole of arterioles but also in the walls of arteries and arterioles far removed from the glomerulus. Typical vascular smooth muscle cells (SMC), in which secretory granules appear, show a concomitant development of their Golgi complex and rough endoplasmic reticulum, with a gradual decrease in the number of their filaments. Microtubules also appear in the Golgi area. Thiery''s periodic acid-thiocarbohydrazide-silver proteinate technique demonstrates that in these intermediate cells, as in mature JGC, the amount of glycogen is greater than in SMC. The newly-developed secretory granules of intermediate cells are stained by phosphotungstic acid at a low pH, as are the mature granules of JGC, an indication that both types contain glycoproteins. Light and electron microscopic autoradiography reveal that both JGC and intermediate cells of the vascular wall do not incorporate radioactive thymidine (injected during the 10 day observation period). They develop by metaplasia of preexistent SMC. In control kidneys, radioactive thymidine is practically never incorporated into the nuclei of SMC but is found in a few glomerular and tubular cells of all zones except the papilla. The endocrine kidney shows virtually no reactive nuclei in vascular SMC, glomeruli, or tubular cells of the outer cortex. Thymidine is incorporated into practically all nuclei of the straight portion of proximal tubules and into about half the nuclei of all medullary tubular cells including the papilla.